Nano MicrovolumesUV-VIS Spectrophotometer, Xenon flash lamp, Fluorescence

SKU: SP-UM100 Category:

Description

1. User-friendly software, easy to use

It is easy to use, free software updates are available

2. Micro-volumes measuring

Only require 0.5~2μl sample to accurate determinate of nucleic acids, proteins

3. Fast and easy measurements

Turn on and instantly measure without lamp warm up time; Easy measurement within 8 sec

4. Long life’s Xenon flash lamp

Xenon flash lamp, 10 flashes, up to 10 years, no dilutions and expensive consumables

5. No consumables required

Direct microvolume measurements from 1–2μL of sample. Eliminates the need for expensive accessories

6. No computer required and with touch screen (SP-UM300, SP-UM400A, SP-UM500)

SP-UM300, SP-UM400A, SP-UM500 is equipped with Android system operation

7. The SP-UM100 / SP-UM300 / SP-UM500 are full range of wavelength (200-800nm) detection ability;

The SPUM400A is a basic UV spectrophotometer specifically designed (only two types of wavelength 260nm and

280nm) for nucleic acids and proteins

8. SP-UM500 also can be used to test the Fluorescence

For samples with concentrations below 2 ng/μl, fluorometric mode can be used, and the minimum detection limit canreach 0.5pg/μl

SP-UM100 Highlights

1. Turn on and instantly measure without lamp warm up time; Full scan capability from 200-800nm within 5 sec

2. Detects Nucleic Acids up to 4500ng/ul (dsDNA)

3. 3864-Element linear silicon CCD array

4. Need to connect computer

5. Software compatibility: WinXP, Win7, Win8

SP-UM300 Highlights

1. SP-UM300 is an improved Micro-Spectrophotometer based on SP-UM100 with 200-800nm. It is added a function of bacterium cell concentration detection (OD600)

2. Android system, 7 inch touch screen, APP software, simple interface, easy to use

3. Equipped with HD touch screen, no need to connect PC

4. 2048-Element linear silicon CCD array detector

5. Turn on and instantly measure without lamp warm up time; Full scan capability from 200-800nm within 6 sec

6. To detect the concentration of bacteria and microorganism are more convenient with the OD600 function

7. The data can be printed with built-in printer, and can output via USB for data analysis and storage

8. When the SP-UM300 is idly left more than 5mins, it will switch off automatically. Then, the user can awake the machine by touching the screen

SP-UM400A Highlights

1. SP-UM400A is a basic micro-volume UV spectrophotometer designed (only 3 types of wavelength: 260nm, 280nm and 365nm) for the measurement of nucleic acids and proteins

2. Android system operation, 7 inch touch screen no computer, APP software, simple interface, easy to use

3. To detect the concentration of bacteria and microorganism are more convenient with the OD600 function

4. Measures nucleic acid concentration at 260nm and purity using the ratio 260/280

5. Measure purified protein concentration at 280nm

6. The data can be printed with built-in printer, the data can output via SD-RAM Card and USB memory

SP-UM500 Highlights

1. SP-UM500 is designed base on SP-UM300:

A. To added the 0.05mm optical path length, and the highestdetection concentration up to 15000ng/ul

B. To added the Fluorescence detection function, which canbe used for accurate quantification of extremely lowconcentration nucleic acid with a lower limit of 0.5pg/μl

C. Automatic detect after put down the arm

2. The patented lifting motor structure makes the liquid columnstretch more gentle, preventing the liquid column from

breaking due to structural problems. In addition, thestructure can effectively solve the instability of readingcaused by high concentration sample, especially suitablefor precise quantification of protein samples

3. Android system, 7-inch capacitive touch screen, intuitiveAPP software, simple and easy to use

4. To detect the concentration of bacteria and microorganismare more convenient with the OD600 function

5. With a 2048-Element linear CCD array detector, the detectionand display can be completed in 6s

6. Equipped with HD touch screen, no need to connect computer.

7. Longer service life of optical component, intelligentidentification of user usage. The light source will auto offafter 5 minutes without any operation to extend service life

8. The result can be printed by a built-in printer or exportedvia USB for data analysis and storage

New fluorescence detection function for SP-UM500

Fluorescence detection combined with fluorescence quantitative analysis kit, able to accurately quantify DNA, RNA and protein concentration through the specific binding of fluorochrome with target material, and the minimum limit is 0.5pg/u(dsDNA). SPUM500 can be compatible with common fluorescence quantitative reagent to provide users with maximum convenience and minimum detection cost.

Nano MicrovolumesUV-VIS Spectrophotometer, Xenon flash lamp, Fluorescence

Fluorescence mode

Code Light ExcitationFilters EmissionFilters
SP-UM500U

(optional)

UV 365±20nm 420-480nm(60nm)
SP-UM500

(standard)

Blue 460±20nm 525-570nm(45nm)
SP-UM500G

(optional)

Green 525±20nm 575-640nm(65nm)
SP-UM500R

(optional)

Red 625±20nm 670-725nm(55nm)

Specification

Light source LED
Dynamic range Fiveordersofmagnitude
Linear Dynamic Range R2>0.995
Detector Photodiode
Repeatability <1.5%
Stability <1.5%
Sensitivity dsDNA: 0.5ng/ml
Measurement speed 3s (Once)

Application of different fluorescence channel

Channel ExcitationFilters Normalkits Applications
UVchannel 365±20nm Hoechst33258,4-MU,EnZCheKCaspase Nuclearacidquantification, Plant GUS reporter genedetection, Poptosisdetection
Bluechannel 460±20nm PicoGreen®,oligreen,RiboGreen®,GFP,Protein,Fluorescein,Quant-iT™ dsDNA, ssDNA, RNAquantification, GFPgene detection,Fluorescein detection, Protein detection
Greenchannel 525±20nm Rhodamine,Cy3,

RFPVybrantCytotoxicity

Rhodamine detection, Cy-3 fluorescencelabeling detection,RFPgene detection, Cellactivityposition detection
Redchannel 625±20nm Cy5,Quant-iTRNA Cy-5 fluorescencelabeling detection, RNAquantification

Fluorescence detection features

FluorescencedetectionisabletoprecisequantificationtheconcentrationofDNA,RNA,proteinwiththehighsensitivefluorescenceanalysiskits.Therelatedfluorochromecouldbeemissiontheopticalwavewhichwavebandislargerthanabsorbinglight,onlywhencombinedwiththetargetmoleculeinthesample,afterabsorbedacertainopticalwave, to quantify  the  intensityof  this  fluorescent.Then,confirmthetargetmaterialcontentsaccordingtothestandard curve

ComparetotheSP-UM300,addedfunctionoffluorescence detectionisavailabletoincreasethelowerlimit(seetheleftdraw)ofsampledetection.Inaddition,eventhereistheRNAexistinginthesamplewhendoingtheDNAdetection,thefluorescencestillwiththestrongspecificity(seetheright draw)

Specifications:

Type SP-UM100
WavelengthRange 200-800nm
MinimumSampleSize 0.5-2.0μl
Path Length 0.2mm1.0mm
LightSource Xenonflashlamp
DetectorType 2048-LinearCCDarray
WavelengthAccuracy 1nm
SpectralResolution ≤3nm(FWHMatHg546nm)
AbsorbancePrecision 0.003Abs
AbsorbanceAccuracy 1%(7.332Absat260nm)
AbsorbanceRange 0.04-90A
DetectsNucleicAcidupto 2-4500ng/ul(dsDNA)
MeasurementTime <5S
DataOutput ConnectPC
SamplePedestalMateria
OperatingVoltage 24VDC
OperatingPower 20W
StandbyPower 5W
Dimension(WxDxH)mm 200x250x166
Weight 2.6kg
SoftwareCompatibility WinXP,Win7,Win8
Sensitivity
LinearDynamicRange
Repeatability
LightSource
WavelengthRange
AbsorbanceRange
Ordering inform ation
No. Description
XY-11060-00 SP-UM500Micro-Spectrophotometer,DC24V5W(Standard)
XY-11070-00 SP-UM500UMicro-Spectrophotometer,DC24V5W(Optional)
XY-11080-00 SP-UM500GMicro-Spectrophotometer,DC24V5W(Optional)
XY-11090-00 SP-UM500RMicro-Spectrophotometer,DC24V5W(Optional)

No.

Code Description

01

XY-11010-00 SP-UM100Micro-Spectrophotometer,DC24V5W

02

XY-11020-00 SP-UM300Micro-Spectrophotometer,DC24V5W

03

XY-11050-00 SP-UM400AMicro-Spectrophotometer,DC24V5W

04

XY-11021-01 CuvetteforSP-UM300,SP-UM400AandSP-UM500

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