Spectrofluorometer

Nano MicrovolumesUV-VIS Spectrophotometer, Xenon flash lamp, Fluorescence

SP-UM400A

More info

Description

1. User-friendly software, easy to use

It is easy to use, free software updates are available

2. Micro-volumes measuring

Only require 0.5~2μl sample to accurate determinate of nucleic acids, proteins

3. Fast and easy measurements

Turn on and instantly measure without lamp warm up time; Easy measurement within 8 sec

4. Long life’s Xenon flash lamp

Xenon flash lamp, 10 flashes, up to 10 years, no dilutions and expensive consumables

5. No consumables required

Direct microvolume measurements from 1–2μL of sample. Eliminates the need for expensive accessories

6. No computer required and with touch screen (SP-UM300, SP-UM400A, SP-UM500)

SP-UM300, SP-UM400A, SP-UM500 is equipped with Android system operation

7. The SP-UM100 / SP-UM300 / SP-UM500 are full range of wavelength (200-800nm) detection ability;

The SPUM400A is a basic UV spectrophotometer specifically designed (only two types of wavelength 260nm and

280nm) for nucleic acids and proteins

8. SP-UM500 also can be used to test the Fluorescence

For samples with concentrations below 2 ng/μl, fluorometric mode can be used, and the minimum detection limit canreach 0.5pg/μl

 

SP-UM100 Highlights

1. Turn on and instantly measure without lamp warm up time; Full scan capability from 200-800nm within 5 sec

2. Detects Nucleic Acids up to 4500ng/ul (dsDNA)

3. 3864-Element linear silicon CCD array

4. Need to connect computer

5. Software compatibility: WinXP, Win7, Win8

 

SP-UM300 Highlights

1. SP-UM300 is an improved Micro-Spectrophotometer based on SP-UM100 with 200-800nm. It is added a function of bacterium cell concentration detection (OD600)

2. Android system, 7 inch touch screen, APP software, simple interface, easy to use

3. Equipped with HD touch screen, no need to connect PC

4. 2048-Element linear silicon CCD array detector

5. Turn on and instantly measure without lamp warm up time; Full scan capability from 200-800nm within 6 sec

6. To detect the concentration of bacteria and microorganism are more convenient with the OD600 function

7. The data can be printed with built-in printer, and can output via USB for data analysis and storage

8. When the SP-UM300 is idly left more than 5mins, it will switch off automatically. Then, the user can awake the machine by touching the screen

SP-UM400A Highlights

1. SP-UM400A is a basic micro-volume UV spectrophotometer designed (only 3 types of wavelength: 260nm, 280nm and 365nm) for the measurement of nucleic acids and proteins

2. Android system operation, 7 inch touch screen no computer, APP software, simple interface, easy to use

3. To detect the concentration of bacteria and microorganism are more convenient with the OD600 function

4. Measures nucleic acid concentration at 260nm and purity using the ratio 260/280

5. Measure purified protein concentration at 280nm

6. The data can be printed with built-in printer, the data can output via SD-RAM Card and USB memory

 

SP-UM500 Highlights

1. SP-UM500 is designed base on SP-UM300:

A. To added the 0.05mm optical path length, and the highestdetection concentration up to 15000ng/ul

B. To added the Fluorescence detection function, which canbe used for accurate quantification of extremely lowconcentration nucleic acid with a lower limit of 0.5pg/μl

C. Automatic detect after put down the arm

2. The patented lifting motor structure makes the liquid columnstretch more gentle, preventing the liquid column from

breaking due to structural problems. In addition, thestructure can effectively solve the instability of readingcaused by high concentration sample, especially suitablefor precise quantification of protein samples

3. Android system, 7-inch capacitive touch screen, intuitiveAPP software, simple and easy to use

4. To detect the concentration of bacteria and microorganismare more convenient with the OD600 function

5. With a 2048-Element linear CCD array detector, the detectionand display can be completed in 6s

6. Equipped with HD touch screen, no need to connect computer.

7. Longer service life of optical component, intelligentidentification of user usage. The light source will auto offafter 5 minutes without any operation to extend service life

8. The result can be printed by a built-in printer or exportedvia USB for data analysis and storage

 

New fluorescence detection function for SP-UM500

Fluorescence detection combined with fluorescence quantitative analysis kit, able to accurately quantify DNA, RNA and protein concentration through the specific binding of fluorochrome with target material, and the minimum limit is 0.5pg/u(dsDNA). SPUM500 can be compatible with common fluorescence quantitative reagent to provide users with maximum convenience and minimum detection cost.

Nano MicrovolumesUV-VIS Spectrophotometer, Xenon flash lamp, Fluorescence

 

Fluorescence mode

Code

Light

ExcitationFilters

EmissionFilters

SP-UM500U

(optional)

UV

365±20nm

420-480nm(60nm)

SP-UM500

(standard)

Blue

460±20nm

525-570nm(45nm)

SP-UM500G

(optional)

Green

525±20nm

575-640nm(65nm)

SP-UM500R

(optional)

Red

625±20nm

670-725nm(55nm)

 

Specification

Light source

LED

Dynamic range

Fiveordersofmagnitude

Linear Dynamic Range

R2>0.995

Detector

Photodiode

Repeatability

<1.5%

Stability

<1.5%

Sensitivity

dsDNA: 0.5ng/ml

Measurement speed

3s (Once)

 

Application of different fluorescence channel

Channel

ExcitationFilters

Normalkits

Applications

UVchannel

365±20nm

Hoechst33258,4-MU,EnZCheKCaspase

Nuclearacidquantification, Plant GUS reporter genedetection, Poptosisdetection

Bluechannel

460±20nm

PicoGreen®,oligreen,RiboGreen®,GFP,Protein,Fluorescein,Quant-iT™

dsDNA, ssDNA, RNAquantification, GFPgene detection,Fluorescein detection, Protein detection

Greenchannel

525±20nm

Rhodamine,Cy3,

RFPVybrantCytotoxicity

Rhodamine detection, Cy-3 fluorescencelabeling detection,RFPgene detection, Cellactivityposition detection

Redchannel

625±20nm

Cy5,Quant-iTRNA

Cy-5 fluorescencelabeling detection, RNAquantification

Fluorescence detection features

FluorescencedetectionisabletoprecisequantificationtheconcentrationofDNA,RNA,proteinwiththehighsensitivefluorescenceanalysiskits.Therelatedfluorochromecouldbeemissiontheopticalwavewhichwavebandislargerthanabsorbinglight,onlywhencombinedwiththetargetmoleculeinthesample,afterabsorbedacertainopticalwave, to quantify  the  intensityof  this  fluorescent.Then,confirmthetargetmaterialcontentsaccordingtothestandard curve

ComparetotheSP-UM300,addedfunctionoffluorescence detectionisavailabletoincreasethelowerlimit(seetheleftdraw)ofsampledetection.Inaddition,eventhereistheRNAexistinginthesamplewhendoingtheDNAdetection,thefluorescencestillwiththestrongspecificity(seetheright draw)

Specifications:


Type

SP-UM400A

WavelengthRange

260nm,280nm

MinimumSampleSize

1-2.0μl

Path Length

0.5mm

LightSource

UVLED

DetectorType

UV-sillionphotocell

WavelengthAccuracy

SpectralResolution

≤8nm(FWHMatHg546nm)

AbsorbancePrecision

0.005Abs

AbsorbanceAccuracy

2%(7.332Absat260nm)

AbsorbanceRange

0.2-50A

DetectsNucleicAcidupto

10-2500ng/ul(dsDNA)

MeasurementTime

<6S

DataOutput

USB

SamplePedestalMateria

AluminumalloyandQu

OperatingVoltage

24VDC

OperatingPower

25W

StandbyPower

5W

Dimension(WxDxH)mm

210x280x181

Weight

2.0kg

SoftwareCompatibility

AndroidSystem

Fluorescentdetection

 

Sensitivity

LinearDynamicRange

Repeatability

OD600nmMeasurement

 

LightSource

LED

WavelengthRange

600±8nm

AbsorbanceRange

0-4A

 

Ordering inform

ation

No.

Description

XY-11060-00

SP-UM500Micro-Spectrophotometer,DC24V5W(Standard)

XY-11070-00

SP-UM500UMicro-Spectrophotometer,DC24V5W(Optional)

XY-11080-00

SP-UM500GMicro-Spectrophotometer,DC24V5W(Optional)

XY-11090-00

SP-UM500RMicro-Spectrophotometer,DC24V5W(Optional)

No.

Code

Description

01

XY-11010-00

SP-UM100Micro-Spectrophotometer,DC24V5W

02

XY-11020-00

SP-UM300Micro-Spectrophotometer,DC24V5W

03

XY-11050-00

SP-UM400AMicro-Spectrophotometer,DC24V5W

04

XY-11021-01

CuvetteforSP-UM300,SP-UM400AandSP-UM500

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